Liquid Chromatography-Mass Spectrometry

What is Liquid Chromatography-Mass Spectrometry?

Liquid chromatography-mass spectrometry (LC-MS) is a combined analytical technique of chromatography and spectrometry used to both separate mixtures of different compounds and identify the molecules. Liquid chromatography separates the mobile phase from the stationary phase molecules. Mass spectrometry determines the structural identity of the compound through mass-to-charge ratio. Using both the techniques, LC-MS is used to analyze different compounds in chemistry.

Why is it important?

LC-MS is commonly used to find the pharmacokinetics of drugs. LC-MS is used to determine the clearance of small molecules in the patients’ body organs. Before introduction of drugs into market, small molecule substances are analyzed using LC-MS to find the half-life and determine the paths taken to be metabolized, distributed and excreted. During drug development stage, LC-MS is used to generate fast analysis of drug weights and structural identification. The technology allows for faster screening of drugs to be ready for the market.

Activity Overview

The activity is for students to finish on their own. The activity aims to give students an opportunity to understand liquid chromatography mass spectrometry (LC-MS). Through completing following activity, the students will be able to fully grasp the concept behind how LC-MS works.

  • Duration: 20 min

  • Materials: worksheet

Class Materials

Below are links to download:

Liquid Chromatography-Mass Spectrometry Info Sheet

Liquid Chromatography-Mass Spectrometry Activity Sheet

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2019 STEmming Edu. 

Dawon Chung 

Yoon Seo Kim

Se Yoon Park